Composite

Part:BBa_J43001:Design

Designed by: Lauren Beatty   Group: iGEM06_Mississippi   (2006-10-12)


J45503.Q04121.E0430


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1566
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

IPTG removes the LAC repressor from LacI+pL site and induces promoter for LacI+pL site. This leads to high levels of YFP transcription. Hydrogen turns on the HybB promoter and LAC repressor is transcribed. The LAC repressor competitively inhibits the LacI+pL site, leading to decreased YFP transcription.

Source

All parts were taken from the registry.

References

hybB promoter was previously used by the UCSF 2005 team.